流沙湾海域勒氏枝鳔石首鱼生物学性状的初步研究

在2013年1—12月渔业资源调查数据的基础上,研究了流沙湾海域勒氏枝鳔石首鱼的体长分布、体长-体质量的关系、肥满度月变化、饵料组成、摄食强度和营养级等生物学特性。结果表明:流沙湾海域枝鳔石首鱼体长82~145 mm,优势体长组为116~125 mm和106~115 mm;体长(L)-体质量(W)关系方程为:W=0.0242L~(2.9564)(R~2=0.9471);肥满度最大值出现在2月份,最小值出现在7月;枝鳔石首鱼的饵料组成以甲壳类为主要类群。枝鳔石首鱼的空胃率随季节变化显著(P0.05),在冬季最大,春季达到最小,饱满度指数在秋季最高,冬季最低;营养级变化范围在2.69~3.11,平均营养级为2.94。     

Comparative study on dietary protein requirements for juvenile and pre‐adult gibel carp (Carassius auratus gibelio var. CAS III)

Two 8‐week growth trials were conducted in indoor recirculation system to evaluate the protein requirements for juvenile (3.70 ± 0.20 g) and pre‐adult (85.2 ± 0.70 g) gibel carp, Carassius auratus gibelio var. CAS III. Six isoenergetic diets were formulated for each trial using fish meal and casein as protein sources, and protein level was 250–450 g kg^?1 in Trial 1 and 200–450 g kg^?1 in Trial 2. With the increasing dietary protein, feeding rate (FR) and feed conversion ratio (FCR) significantly decreased (P < 0.05). Weight gain (WG) increased first and then reached a plateau in 330–450 g kg^?1 in Trial 1 (P > 0.05), while decreased after the maximum value in 350 g kg^?1 in Trial 2 (P < 0.05). Productive protein values (PPVs) were lower in 370–450 g kg^?1 in Trial 1 and 400–450 g kg^?1 in Trial 2 (P < 0.05). Increasing dietary protein level increased protein content and decreased lipid content in whole fish body and white muscle (P < 0.05). Apparent digestibility coefficient of dry matters (ADC_d) decreased, while apparent digestibility coefficient of protein (ADC_p) increased in 370–450 g kg^?1 in Trial 1 and 250–450 g kg^?1 in Trial 2 (P < 0.05). Trypsin activity significantly increased in 370–450 g kg^?1 in Trial 1 (P < 0.05) and was not affected in Trial 2 (P > 0.05). Hepatic alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in both trials increased when dietary protein was above 400 g kg^?1 (P < 0.05). Based on quadratic regression of WG, it was estimated that dietary protein requirement for maximum growth was 414 g kg^?1 (digestible protein of 376 g kg^?1) and 365 g kg^?1 (digestible protein of 324 g kg^?1) for juvenile (3.70 g) and pre‐adult gibel carp (85.2 g).     

利用CRISPR/Ca9技术靶向编辑芥蓝BoaZDS

以芥蓝(Brassicaoleraceavar.alboglabra)为材料,以ζ–胡萝卜素脱氢酶(ζ-Carotene desaturase,ZDS)基因为目标基因,建立其CRISPR/Cas9基因组编辑体系。在BoaZDS的编码区近5′端选择靶位点,构建了CRISPR/Cas9表达载体,通过农杆菌介导的遗传转化方法获得了19个芥蓝转基因阳性植株,Sanger测序分析发现其中13株成功突变,CRISPR/Cas9载体在芥蓝上的突变效率为68.42%,且所有突变植株均表现出明显的白化表型。     

食品安全国家标准GB 2763—2016对葡萄的新要求

《食品安全国家标准 食品中农药最大残留限量》(GB 2763—2016)对食品中农药残留的种类、最大残留限量及检测方法均提出了较多的新要求。对新标准中涉及葡萄或浆果类水果规定的变化进行了归纳,并与国际上的相关标准进行对比,旨在为有关产业的发展提供参考。     

气候变化情景下毛竹潜在分布及动态预测

为探究毛竹的潜在分布及其动态变化，并确定对其分布起主导作用的环境变量，文章基于MaxEnt预测模型，利用环境变量图层及316个毛竹分布点数据研究毛竹地理分布的变化。结果显示：1）限制毛竹分布的主要环境变量为最干月降水量、年均降水量、最冷月最低温、年平均温、温度年较差和海拔；2）当前毛竹的潜在适生区主要位于中国东南部，与亚热带季风气候区大致重叠，高适生区主要位于总适生区的东部和西北部；3）在未来气候情景下，毛竹总适生区面积有所缩减，各不同等级适生区面积变化大，其中高适生区面积大幅度缩减。研究获得的毛竹适生区变化趋势可为毛竹培育、引种以及入侵防治提供参考。     

不同氮肥水平下烟草CO2响应曲线的拟合分析

[目的]探讨不同CO2响应曲线模型拟合不同氮肥水平下烟草光合特性与CO2间的关系,筛选出最佳的CO2响应曲线拟合模型.[方法]以烤烟K326为对象,在不同氮肥用量(0、66.60、93.60和120.60 kg/ha)条件下,采用不同CO2响应曲线模型(直角双曲线模型、Michaelis-Menten模型、直角双曲线修正模型和指数改进模型),对烟株旺长期中部叶的CO2响应曲线进行拟合,分析拟合曲线与实测值的相似程度,筛选拟合效果最佳模型.[结果]直角双曲线模型、Michaelis-Menten模型和指数改进模型均无法完全拟合出光合速率随CO2增加而下降的曲线,且直角双曲线模型和Michaelis-Menten模型无法拟合最大净光合速率(Pn max).直角双曲线修正模型拟合R2为0.999,拟合效果优于其他模型,其光合参数[初始羧化效率(α)、Pn max和饱和胞间CO2浓度(Ci sat)]的拟合值更接近真实值,能反映实际情况.各模型决定系数排序为直角双曲线修正模型>指数改进模型>直角双曲线模型=Michaelis-Menten模型.[结论]不同CO2响应曲线模型对不同氮水平下烟草光合特性的拟合效果存在差异,四种模型中以直角双曲线修正模型的拟合效果最佳.     

玉米XYLPs基因家族表达模式及其蛋白结构分析

通过生物信息学的方法对玉米木质形成素类阿拉伯半乳糖蛋白(xylogen-like arabinogalactan proteins,XYLPs)基因家族的表达模式和蛋白结构进行了分析。ZmXYLPs基因家族成员在染色体上的分布具有一定的偏好性。基因复制事件不仅在基因家族成员数量的扩张和进化中发挥重要作用,同时还可能引起基因表达部位的扩张。根据ZmXYLPs蛋白结构特点,可将其分为两大类。其中仅ZmXYLP10/13/14蛋白存在N连接的糖修饰位点,其余ZmXYLPs蛋白均为O连接糖修饰位点。结果显示,玉米ZmXYLPs的生物学功能可能与其糖基侧链有关,并且其作用并不局限于维管组织发生过程,可能也参与生殖发育过程。     

有机肥配施生物刺激素对土壤微生物和番茄生长及产量的影响

探讨有机肥配施生物刺激素对土壤微生物、植株长势及产量的影响,为植物生长提供优化的施肥方案。以番茄(Solanum lycopersicum)为试验材料,田间小区试验采用随机区组设计,设置4个处理,分别为对照(CK)、细沙配施0.1%生物刺激素、福邦5%有机肥、福邦5%有机肥配施0.1%生物刺激素。检测了各试验小区土壤微生物的变化情况、番茄植株的长势和产量等数据,统计分析了各处理间差异。结果表明,5%有机肥配施0.1%生物刺激素处理能显著提高土壤中真菌、细菌、放线菌的数量;番茄植株的长势、产量明显高于其他3个处理。由此可知,5%有机肥配施0.1%生物刺激素能够显著提高土壤有益微生物种群,促进植物生长,既能提高蔬菜产量,又能改善品质。     

Effect of dietary Moringa stem meal level on growth performance,slaughter performance and serum biochemical parameters in geese

Moringa stem meal (MSM) with a high level of crude fibre (CF) might be developed and utilized in herbivorous geese as an unconventional feedstuff. The aim of this study was to investigate the effect of the MSM level in the diet on the growth performance, slaughter performance, breast meat quality and serum biochemical parameters in geese from 22 to 70 days of age. A one-factor completely randomized design was adopted in our study. A total of one thousand eight 21-day-old geese were randomly divided into six groups, with six replicates per group and 28 birds per replicate. The geese were fed diets containing MSM levels of 0, 20, 40, 60, 80 or 100 g/kg during day 22–70. The dietary MSM level had no effect (p > .05) on the final body weight (BW), average daily gain (ADG) or average daily feed intake (ADFI). The feed/gain ratio (F/G) increased linearly (p < .001) as the dietary MSM level increased. No differences (p > .05) were observed in the slaughter performance, meat quality and the relative organ weight (except for thymus) of the geese (p > .05). The relative weight of the thymus in the geese fed diets with supplementation of MSM was higher than that in the non-supplemented MSM control group (p < .05). In addition, 100 g MSM/kg of diet decreased the serum glucose (GLU) level (p < .05) and increased the alanine transaminase (ALT) enzyme activity (p = .03). Dietary MSM levels of no more than 60 g/kg had no effects on the growth performance and slaughter performance, whereas diets with 100 g MSM/kg increased the F/G and serum ALT enzyme activity, as well as decreasing the serum GLU level. Therefore, MSM provided at a reasonable level could be developed as an unconventional feedstuff for geese at the finisher period.